荷兰阿姆斯特丹大学博士后——植物分子生物学方向

荷兰阿姆斯特丹大学博士后——植物分子生物学方向

阿姆斯特丹大学（荷文：Universiteit van Amsterdam；英文：University of Amsterdam，简称UvA）创建于1632年，是一所坐落在荷兰首都阿姆斯特丹的研究型大学。它是全球大学高研院联盟、Universitas 21、欧洲首都大学联盟、欧洲研究型大学联盟成员。学校共诞生了六位诺贝尔奖获得者，五位荷兰首相，七位斯宾诺莎奖得主和一位图灵奖得主。知名校友包括欧洲理事会主席夏尔·米歇尔，Python之父吉多·范罗苏姆，图灵奖得主艾兹格·迪科斯彻，诺贝尔物理学奖得主范德华和世界国际象棋联合会主席马克斯·尤伟等。

Postdoc in Plant Molecular Biology to Unravel Volatile Communication in Plants

UvA - University of Amsterdam

Amsterdam, Netherlands

What you will do

In this interdisciplinary project, you will build upon groundbreaking discoveries in the field of "green leaf volatiles" (GLVs) – a group of compounds released by plants upon herbivory. Our team has uncovered a class of enzymes in plants and insects (called hexenal isomerases) that have significant influence on ecological dynamics by re-arranging the green leaf volatile, Z-3-hexenal, into E-2-hexenal. We have shown that these changes affect not only the behavior of insects interacting with the plant but also the metabolism of the plant itself as well as its nearby competitors.

Research Objectives:

Together with the PhD student you will engineer non-responsive and non-producing E-2-hexenal mutant potato plants. This will allow you to explore the profound roles E-2-hexenal plays in self-recognition, interactions with herbivorous insects, and responses to pathogens. Additionally you will start a new project studying the role of GLVs in promoting plant fitness through predator attraction.

During your project, you will apply a variety of techniques, including (but not limited to) DNA cloning, creation of mutant plants using CRISPR/Cas9 technology, standard Plant Physiology measurements to phenotype mutant plants, Bioassays with several pests and pathogens, RNAseq, metabolomics including GC-MS and LC-MS analyses.

Tasks and responsibilities:

create mutant potato plants impaired in E-2-hexenal perception or hexenal isomerase (HI) activity using CRISPR/Cas9-technologies;

validate the effect of mutations (in HI and receptor candidates) on the physiology of plants and on their ability to interact with their environment;

generate GLV producing Arabidopsis plants by prime editing and perform choice experiments, headspace analysis and fitness experiments;

perform your experiments in a systematic and well controlled manner and keep accurate records by properly documenting and organizing your work;

be an active member of the research group and take responsibility for shared tasks. Discuss your work with the group members and other members of the UvA Plant Science community. Incorporate feedback and give input to others.

take a leading role in writing manuscripts.

What we look for

You are passionate about science and have a particular interest in plant molecular biology with a special focus on biotic interactions. You enjoy performing experimental lab work (e.g., molecular biology, analytical chemistry using GC-MS and LC-MS, plant transformation and bioassays with pests and pathogens). You have a creative mind and look forward to work at the cutting-edge of technology. Finally, you are a team player and a pleasant colleague who enjoys being part of an interdisciplinary team of plant scientists.

You have/are:

a PhD degree, preferably in molecular (plant) sciences or a related area in molecular biology, plant physiology or pathology;

demonstrated hands-on experience with at least several of the following techniques: DNA cloning, Virus-induced gene silencing and/or CRISPR/Cas in potato and Arabidopsis, chemical ecology, plant tissue culture, bioassays with herbivores such as aphids, spider mites etc.;

fluency in English, both written and spoken.

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